Enzyme Immunoassay for the Quantitative Determination of Estradiol (E2) Concentration in
Human Serum or Plasma
STORAGE OF TEST KIT AND INSTRUMENTATION
Unopened test kits should be stored at 2-8°C upon receipt and the microtiter plate should be kept in a sealed bag with desiccants to minimize exposure to damp air. Opened test kits will remain stable until the expiration date shown, provided it is stored as described above. A microtiter plate reader with a bandwidth of 10 nm or less and an optical density range of 0-3 O.D. at 450 nm wavelength is acceptable for use in absorbance measurement.
Test: Estradiol (E2) ELISA
Method: ELISA: Enzyme Linked Immunosorbent Assay
Principle: Indirect: Antigen Coated
Detection Range: 0-1000 pg/ml
Sample: 25ul serum
Specificity: 100%
Sensitivity: 10pg/ml
Total Time: ~ 110 min
Shelf Life: 12-14 months
| Materials provided with the kit: • Goat Anti-Rabbit IgG-coated microtiter wells, 96 wells • Estradiol Reference Standards: 0, 10, 30, 100, 300, and 1000 pg/ml. Liquid, 0.5 ml each, ready to use. • Rabbit Anti-Estradiol Reagent (pink color), 7 ml • Estradiol-HRP Conjugate Reagent (blue color), 12 ml • Estradiol Control 1, Liquid, 0.5 ml, Ready to use. • Estradiol Control 2, Liquid, 0.5 ml, Ready to use. • TMB Reagent (One-Step), 11 ml. • Stop Solution (1N HCl), 11 ml. | Materials required but not provided: • Precision pipettes: 25 μl , 50 μl, 100 μl, 200 μl, and 1.0 ml. • Disposable pipette tips. • Distilled and deionized water. • Vortex mixer or equivalent. • Absorbent paper or paper towel. • Linear-linear graph paper. • Microtiter plate reader. |
