Testosterone Enzyme Immunoassay
For the quantitative determination of Testosterone concentration in human serum
Storage of Test Kit and Instrumentation
Unopened test kits should be stored at 2-8°C upon receipt and the microtiter plate should be kept in a sealed bag with desiccants to minimize exposure to damp air. Opened test kits will remain stable
until the expiration date shown, provided it is stored as described above. A microtiter plate reader with a bandwidth of 10 nm or less and an optical density range of 0-3 O.D. at 450 nm wavelength is acceptable for use in absorbance measurement.
Test: Testosterone
Method: Enzyme Linked Immunosorbent Assay
Principle: Peroxidase – Conjugated Competitive ELISA
Detection Range: 0-18 ng/ml
Sample: 10ul serum
Specificity: 100%
Sensitivity: 0.05 ng/ml
Total Time: ~ 110 min
Shelf Life: 12-14 months
| Materials provided with the kit: • Goat Anti-Rabbit IgG-coated microtiter wells, 96 wells • Testosterone Reference Standards: 0, 0.1, 0.5, 2.0, 6.0 and 18.0 ng/ml. Liquids, 0.5 ml each, ready to use. • Rabbit Anti-Testosterone Reagent (pink color), 7 ml • Testosterone-HRP Conjugate Reagent (blue color), 12 ml • Testosterone Control 1, Liquid, 0.5 ml, Ready to use. • Testosterone Control 2, Liquid, 0.5 ml, Ready to use. • TMB Reagent (One-Step) 11 ml. • Stop Solution (1N HCl), 11 ml. | Materials required but not provided: • Precision pipettes: 10 μl, 50 μl, 100 μl, and 1.0 ml. • Disposable pipette tips. • Distilled or deionized water. • Vortex mixer or equivalent. • Absorbent paper or paper towel. • Linear-linear graph paper. • Microtiter plate reader. |
