PVDF-Plus is a naturally hydrophobic, unsupported polyvinylidene fluoride transfer membrane designed to deliver the highest binding capacity combined with low backgrounds in western transfer, amino acid analysis and protein sequencing applications. Exceptional quality control procedures throughout the manufacturing process eliminates lot-to-lot variation and minimizes bald spot problems. PVDF-Plus assures reproducible results with maximum sensitivity.
Protein can be electroblotted from a variety of gel matrices onto PVDF-Plus. Because of its high sensitivity and strong binding affinity, protein will not pass through the membrane. The chemical compatibility of PVDF-Plus allows the use of all commonly used stains such as: Amino Black, Colloidal Gold, Coomassie Blue, India Ink and Ponceau-S. PVDF-Plus will not degrade, distort or shrink when using high concentrations of methanol for destaining.
PVDF-Plus exhibits efficient transfer of proteins in a wide range of molecular weights, from 5k daltons to 700k Daltons. The binding capacity averages 125 ?g/cm2 for larger globular proteins such as immunoglobulins and higher binding capacities for small peptides.
Protein can be electroblotted from a variety of gel matrices onto PVDF-Plus. Because of its high sensitivity and strong binding affinity, protein will not pass through the membrane. The chemical compatibility of PVDF-Plus allows the use of all commonly used stains such as: Amino Black, Colloidal Gold, Coomassie Blue, India Ink and Ponceau-S. PVDF-Plus will not degrade, distort or shrink when using high concentrations of methanol for destaining.
PVDF-Plus exhibits efficient transfer of proteins in a wide range of molecular weights, from 5k daltons to 700k Daltons. The binding capacity averages 125 ?g/cm2 for larger globular proteins such as immunoglobulins and higher binding capacities for small peptides.
